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Pablo Villanueva Perez

Senior lecturer

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Dose-efficient scanning Compton X-ray microscopy

Author

  • Tang Li
  • J. Lukas Dresselhaus
  • Nikolay Ivanov
  • Mauro Prasciolu
  • Holger Fleckenstein
  • Oleksandr Yefanov
  • Wenhui Zhang
  • David Pennicard
  • Ann Christin Dippel
  • Olof Gutowski
  • Pablo Villanueva-Perez
  • Henry N. Chapman
  • Saša Bajt
Show all

Summary, in English

The highest resolution of images of soft matter and biological materials is ultimately limited by modification of the structure, induced by the necessarily high energy of short-wavelength radiation. Imaging the inelastically scattered X-rays at a photon energy of 60 keV (0.02 nm wavelength) offers greater signal per energy transferred to the sample than coherent-scattering techniques such as phase-contrast microscopy and projection holography. We present images of dried, unstained, and unfixed biological objects obtained by scanning Compton X-ray microscopy, at a resolution of about 70 nm. This microscope was realised using novel wedged multilayer Laue lenses that were fabricated to sub-ångström precision, a new wavefront measurement scheme for hard X rays, and efficient pixel-array detectors. The doses required to form these images were as little as 0.02% of the tolerable dose and 0.05% of that needed for phase-contrast imaging at similar resolution using 17 keV photon energy. The images obtained provide a quantitative map of the projected mass density in the sample, as confirmed by imaging a silicon wedge. Based on these results, we find that it should be possible to obtain radiation damage-free images of biological samples at a resolution below 10 nm.

Department/s

  • LU Profile Area: Light and Materials
  • LTH Profile Area: Photon Science and Technology
  • LTH Profile Area: Nanoscience and Semiconductor Technology
  • NanoLund: Centre for Nanoscience
  • Synchrotron Radiation Research

Publishing year

2023

Language

English

Publication/Series

Light: Science and Applications

Volume

12

Issue

1

Document type

Journal article

Publisher

Nature Publishing Group

Topic

  • Radiology and Medical Imaging

Status

Published

ISBN/ISSN/Other

  • ISSN: 2095-5545