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Pablo Villanueva Perez

Senior lecturer

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Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline

Author

  • Simone Sala
  • Yuhe Zhang
  • Nathaly De La Rosa
  • Till Dreier
  • Maik Kahnt
  • Max Langer
  • Lars Dahlin
  • Martin Bech
  • Pablo Villanueva Perez
  • Sebastian Kalbfleisch

Summary, in English

X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive (e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide complementary information at a comparable resolution, ultimately limited by the focal spot size. The experimental setup presented allows the user to swap between them in a flexible and reproducible fashion, as well as to easily adapt the scanning parameters during an experiment to fine-tune resolution and field of view.

Department/s

  • MAX IV Laboratory
  • Synchrotron Radiation Research
  • NanoLund: Centre for Nanoscience
  • Medical Radiation Physics, Lund
  • EpiHealth: Epidemiology for Health
  • WCMM-Wallenberg Centre for Molecular Medicine
  • Department of Translational Medicine
  • Hand Surgery, Malmö
  • X-ray Phase Contrast

Publishing year

2022-03-16

Language

English

Publication/Series

Journal of Synchrotron Radiation

Volume

29

Issue

3

Document type

Journal article

Publisher

International Union of Crystallography

Topic

  • Atom and Molecular Physics and Optics
  • Radiology and Medical Imaging
  • Subatomic Physics

Status

Published

Research group

  • Hand Surgery, Malmö
  • X-ray Phase Contrast

ISBN/ISSN/Other

  • ISSN: 1600-5775